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Worsening macrocytic anemia : B 12 deficiency may also cause a macrocytic (megaloblastic) anemia BUT, in the presence of folate supplementation, this anemia may be masked so that B 12 deficiency may present with exclusively neurologic features

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For immunohistochemistry, detection was performed using a biotinylated anti-rabbit IgG secondary antibody and streptavidin-Horseradish peroxidase (HRP), followed by colorimetric detection using DAB